Unresponsiveness to Recombinant Hepatitis B Vaccine in Healthy Iranian Neonates: Association with HLA Antigens
نویسندگان
چکیده
Background: Hepatitis B is an important infectious disease. Since several years ago, mass vaccination against this viral infection has become as part of routine vaccination schedule of Iran. However, some healthy neonates, children and adults fail to generate a protective antibody response after vaccination. Objectives: To investigate distribution of HLA class-I and class-II antigens in healthy Iranian neonates vaccinated with recombinant hepatitis B vaccine. Methods: HLA-typing was performed on peripheral blood lymphocytes isolated from 25 responder and 23 nonresponder (anti-HBs < 10 IU/L) healthy neonates, using the standard microlymphocytotoxicity method. Anti-HBs antibody was quantitated by sandwich ELISA. Results: The frequency of HLA-DR7 (p<0.01), DQ2 (p<0.02) and DR13 (p<0.05) was significantly higher in the nonresponder neonates compared to the responder group. The DR1 and DQ3 antigens were over-represented (p<0.05) in the responder vaccinees, implying positive association with the anti-HBs antibody response. Statistical analysis revealed increased frequencies of B7-DR7-DR53-DQ2 and DR13-DR52-DQ2 haplotypes in the nonresponder neonates
منابع مشابه
Unresponsiveness to Recombinant Hepatitis B Vaccine in Healthy Iranian Neonates: Association with HLA Antigens
Background: Hepatitis B is an important infectious disease. Since several years ago, mass vaccination against this viral infection has become as part of routine vaccination schedule of Iran. However, some healthy neonates, children and adults fail to generate a protective antibody response after vaccination.Objectives: To investigate distribution of HLA class-I and class-II antigens in health...
متن کاملDownregulation of IL-12 Production in Healthy Non-Responder Neonates to Recombinant Hepatitis B Vaccine
A proportion of healthy neonates and adults fail to develop a protective antibody response to recombinant hepatitis B (HB) vaccine. Unresponsiveness to vaccination could be attributed to defect in a number of immunological regulatory mechanisms. In this study, IL-12 was quantitated in culture supernatant following in vitro stimulation of peripheral blood mononuclear cells isolated from a group ...
متن کاملThe factors influencing the immune response to hepatitis B vaccine and persistence of the protection.
Hepatitis B virus (HBV) infection and its sequelae which include cirrhosis and hepatocellular carcinoma is a major public health problem throught the world.The WHO strategy for effective control of HBV infection is vaccination with the surface antigen of virus(HBsAg).The results obtained from a large number of studies demonstrated that the vaccine induces a protective antibody resonse (anti-HB...
متن کاملDifferential Immunogenicity of A Recombinant Hepatitis B Vaccine in Iranian Neonates: Influence of Ethnicity and Environmental Factors
Objective: To compare immunogenicity of a recombinant hepatitis B (HB) vaccine in two groups of neonates born in two cities of Iran with different geographic and ethnic backgrounds. Materials and Methods: Ten micrograms of a recombinant HB vaccine was administered under field condition to Iranian healthy neonates at 0, 1.5 and 9 months intervals. The subjects consisted of two groups of 290 an...
متن کاملDistribution of Circulating Immune Cells in Responder and Non-Responder Individuals to Hepatitis B Vaccine
Unresponsiveness to hepatitis B surface antigen (HBsAg) has been shown to be associated with dysfunction of the presenting cells (APC) and defect in the specific B-lymphocyte and/or T-lymphocyte repertoires. Direct determination of the frequency of specific T-lymphocytes together with complementary analysis of the naive circulating immune cells could provide valuable information about the cel...
متن کامل